兰轲 教授（博士生导师）

兰轲 教授（博士生导师）

单 位：四川大学华西药学院

通讯地址：成都市人民南路三段17号

电子邮件：lanwoco@scu.edu.cn

简历

2024.09~今 四川大学华西药学院，教授

2015.03~2016.02，夏威夷大学癌症研究中心，访问学者

2011.07~2024.08，四川大学华西药学院，副教授

2007.07~2011.06，四川大学华西药学院，讲师

2004.09~2007.06，四川大学华西药学院，生物药剂学，博士研究生

2001.09~2004.06，四川大学华西药学院，生物药剂学，硕士研究生

1996.09~2001.06，四川大学华西药学院，临床药学，本科

学术任职与荣誉

· 2019~今：美国药理学与实验治疗学会 (ASPET)会员

· 2018~今：中国药理学会药物代谢专业委员会青年委员

· 2018：Young Scientist Awards, 6^th APISSX, Zhejiang University, Hangzhou

· 2017：第三届刘昌孝人才奖“优秀青年科技工作者奖（提名奖）”

学术刊物任职

· 2021~今：Review Editor for Translational Pharmacology, Frontiers in Pharmacology

· 2017~今：《中国临床药理学杂志》青年编委

· 2015~今：Editorial Board Member, Eur J Drug Metab Pharmacokinet

研究方向

· 胆汁酸代谢

· 药物代谢

· 药物代谢组学

主要研究课题及成果

主要研究课题

· 国家自然科学基金面上项目，CYP8B1催化胆汁酸合成中间体12位氧化代谢机理的应用基础研究，2024.1~2027.12，在研

· 国家自然科学基金面上项目，CYP3A在人类胆汁酸宿主-肠道微生物协同代谢中的生物学功能研究及其药物代谢组学转化探究，2021.1~2024.12，已结题

· 国家自然科学基金青年科学基金项目，运用代谢组学技术系统研究乌头碱类镇痛药的体内活性/毒性代谢产物，2014.1-2016.12，已结题

授权专利

1. 兰轲，桂兰兰，一种检测细胞色素CYP8B1酶活性的方法，专利号：ZL 202210929229.8，

2. 兰轲，徐亮，张建，黄亮，细胞色素CYP3A7酶的新型特异性探针反应及其应用，专利号：ZL 2018 1 0953793.7

3. 兰轲，徐亮，黄亮，细胞色素CYP3A7酶的特异性探针反应及其应用，专利号：ZL 2018 1 0543953.0

代表性科研论文

1. Wang YT, Wang YX, Hu YT, Wu QL, Gui LL, Zeng WS, Chen Q, Yu TT, Zhang XJ, Lan K*. CYP8B1 catalyzes 12alpha-hydroxylation of C27 bile acid from dihydroxycoprostanic acid into trihydroxycoprostanic acid. Drug Metab Dispos, 2024, 52(11): 1234-1243.

2. Chang YB, Li XJ, Jiang JP, Gui LL, Wan LF, Zhou XX, Liao LC*,1, Li KX*, Lan K*. Separation of bile acid isomer plays a pivotal role in bioequivalence evaluation of ursodeoxycholic acid.  J Pharm Biomed Anal. 2024, 239:115882.

3. Wang CT, Cheng B, Wei W, Gui LL, Zeng WS, Wang YT, Wang YX, Chen Q, Xu L, Miao J*, Lan K*. Comparison of 1beta- and 5beta-hydroxylation of deoxycholate and glycodeoxycholate as in vitro index reactions for CYP3A activities. Drug Metab Dispos, 2024, 52(2):126-134.

4. Huang L, Wei W, Huang XM, Li XJ, Liu HS, Gui LL, Jiang JP, Wan LF, Zhou XX, Ding JS, Jiang XH, Zhang BK*, Lan K*. High fat diets enhance and delay ursodeoxycholic acid absorption but elevate circulating hydrophobic bile salts. Front Pharmacol, 2023, 14: 1168144.

5. Wu QL, Hu YT, Wang CT, Wei W, Gui LL, Zeng WS, Liu CX, Jia W, Miao J, Lan K*. Reevaluate in vitro CYP3A index reactions of benzodiazepines and steroids between humans and dogs. Drug Metab Dispos, 2022, 50(6):741-749.

6. Gui LL, Wu QL, Hu YT, Zeng WS, Tan XW, Zhu PP, Li XJ, Yang L, Jia W, Liu CX, Lan K*. Compensatory transition of bile acid metabolism from fecal disposition of secondary bile acids to urinary excretion of primary bile acids underlies rifampicin-induced cholestasis in beagle dogs. ACS Pharmacol Transl Sci, 2021, 4(2):1001-1013.

7. Zeng WS, Gui LL, Tan XW, Zhu PP, Hu YT, Wu QL, Li XJ, Yang L, Jia W, Liu CX, Lan K*. Tertiary oxidation of deoxycholate is predictive of CYP3A activity in dogs. Drug Metab Dispos, 2021, 49(5): 369-378.

8. 丁劲松, 王安娜, 黄亮, 贾伟, 刘昌孝, 兰轲*. 人体熊去氧胆酸代谢及其生物等效性研究的技术挑战. 药学学报, 2020, 55(9): 2070-2079.

9. Wang WX, Chen L, Wang GY, Zhang JL, Tan XW, Lin QH, Chen YJ, Zhang J, Zhu PP, Miao J, Su MM, Liu CX, Jia W, Lan K*. Urinary bile acids profile of cesarean sectioned newborns is characterized by oxidative metabolism of primary bile acids: limited roles of fetal-specific CYP3A7 in cholate oxidations. Drug Metab Dispos, 2020, 48 (8) 662-672.

10. Lin  QH, Tan XW, Wang WX, Zeng WS, Gui LL, Su MM, Liu CX, Jia W, Xu L, Lan K*. Species differences of bile acid redox metabolism: tertiary oxidation of deoxycholate is conserved in preclinical animals. Drug Metab Dispos, 2020, 48 (6) 499-507.

11. 兰轲. 药物代谢酶CYP3A在胆汁酸宿主－肠道微生物协同代谢中的生物学功能假说. 中国临床药理学杂志. 2019, 35(22):2923-2929.

12. Chen YJ, Zhang J, Zhu PP, Tan XW, Lin QH, Wang WX, Yin SS, Gao LZ, Su MM, Liu CX, Xu L, Jia W, Sevrioukova IF*, Lan K*. Stereoselective oxidation kinetics of deoxycholate in recombinant and microsomal CYP3A enzymes: deoxycholate 19-hydroxylation is an in vitro marker of CYP3A7 activity. Drug Metab Dispos, 2019, 47 (6) 574-581.

13. Zhang J, Gao LZ, Chen YJ, Zhu PP, Yin SS, Su MM, Ni Y, Miao J, Wu WL, Chen H, Brouwer KLR, Liu CX, Xu L, Jia W*, Lan K*. Continuum of host-gut microbial co-metabolism: CYP3A4/3A7 are responsible for tertiary oxidations of deoxycholate species. Drug Metab Dispos, 2019, 47(3): 283-294.

14. Zhu P, Zhang J, Chen Y, Yin S, Su M, Xie G, Brouwer KLR, Liu C, Lan K*, Jia W*. Analysis of human C24 bile acids metabolome in serum and urine based on enzyme-digestion of conjugated bile acids and LC-MS determination of unconjugated bile acids. Anal Bioanal Chem. 2018, 410(21): 5287-5300.

15. Yin S, Su M, Xie G, Li X, Wei R, Liu C, Lan K*, Jia W*. Factors affecting separation and detection of bile acids by liquid chromatography coupled with mass spectrometry at negative mode. Anal Bioanal Chem. 2017, 409(23):5533-5545. doi: 10.1007/s00216-017-0489-1

16. Lan K, Su M, Xie G, Ferslew BC, Brouwer KL, Rajani C, Liu C, Jia W*. Key role of 12-hydroxy group for the negative ion fragmentation of unconjugated C24 bile acids. Anal Chem, 2016, 88 (14): 7041-7048.